Journal: Journal of Cancer

Article Title: Oridonin inhibits tumor angiogenesis and induces vessel normalization in experimental colon cancer

doi: 10.7150/jca.55929

Figure Lengend Snippet: ORI inhibited angiogenesis of colon cancer. (A) Representative images of immunohistochemical analysis of tumor microvessels (×200, scale bar =100 µm). (B) CD31 + MVD was dramatically suppressed in ORI- treated group compared to the Control group. (C) Representative Western blot expressions of VEGF, bFGF, angiostatin and endostatin. (D and E) The levels of VEGF and bFGF were dramatically down-regulated in the ORI-treated group, while (F and G) angiostatin and endostatin were increased by treatment with ORI. * p < 0.05, ** p < 0.01.

Article Snippet: The membranes were incubated with primary antibodies as follows: rabbit anti-JAK2 (1:1000, Cell Signaling Technology), or rabbit anti-p-JAK2 (1:1000, Cell Signaling Technology), or mouse anti-STAT3 (1:1000, Cell Signaling Technology), or mouse anti-p-STAT3 (1:2000, Cell Signaling Technology), or rabbit anti-β-actin (1:6000, Abcam), or rabbit anti-VEGF (1:1000, Servicebio), or rabbit anti-bFGF (1:1000, Bioworld), or rabbit anti-angiostatin (1:1000, Bioworld), or rabbit anti- endostatin (1:1000, Boster), with gentle shaking at 4 °C overnight.

Techniques: Immunohistochemical staining, Control, Western Blot

Journal: Oncology Letters

Article Title: Efficacy of pEgr-1-endostatin combined with ionizing radiation on hypoxic conditions in nude mice bearing SKOV3 ovarian carcinoma

doi: 10.3892/ol.2017.5559

Figure Lengend Snippet: Primers for polymerase chain reaction amplification.

Article Snippet: The membranes were blocked with PBS containing 1% Tween 20 at 4°C overnight, washed and incubated overnight at 4°C with anti-HIF-1α (#AH339-2) and anti-endostatin (#BAF1098) primary antibodies (1:1,000 dilution in TBS-T; Bioworld Technology, Inc., St. Louis Park, MN, USA).

Techniques: Polymerase Chain Reaction, Amplification, Sequencing

Journal: Oncology Letters

Article Title: Efficacy of pEgr-1-endostatin combined with ionizing radiation on hypoxic conditions in nude mice bearing SKOV3 ovarian carcinoma

doi: 10.3892/ol.2017.5559

Figure Lengend Snippet: Expression of endostatin in the three groups. (A) Agarose gel electrophoresis results of endostatin mRNA expression, as detected by reverse transcription-polymerase chain reaction in the different groups. (B) The relative mRNA level of endostatin was examined after group C had been injected with pEgr-1-endostatin plasmid for 12 h and exposed to 300 cGy/min X-ray for 48 h. Endostatin was successfully increased in the group C compared with that in groups A and B (F=380.078, P<0.001). (C) The protein level of endostatin was evaluated by western blotting. The results indicated that the expression of endostatin was significantly increased in group C compared with groups A and B (P=0.039). (D) ELISA was used to determine the plasma endostatin level, and the expression of endostatin in group C was observed to be higher than that in groups A (t=44.770, P<0.001) and B (t=42.480, P<0.001). A, control group; B, IR-treated group; C, endostatin-IR-treated group; IR, ionizing radiation; M, marker; mRNA, messenger RNA. ***P<0.001.

Article Snippet: The membranes were blocked with PBS containing 1% Tween 20 at 4°C overnight, washed and incubated overnight at 4°C with anti-HIF-1α (#AH339-2) and anti-endostatin (#BAF1098) primary antibodies (1:1,000 dilution in TBS-T; Bioworld Technology, Inc., St. Louis Park, MN, USA).

Techniques: Expressing, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Injection, Plasmid Preparation, Western Blot, Enzyme-linked Immunosorbent Assay, Marker

Journal: Oncology Letters

Article Title: Efficacy of pEgr-1-endostatin combined with ionizing radiation on hypoxic conditions in nude mice bearing SKOV3 ovarian carcinoma

doi: 10.3892/ol.2017.5559

Figure Lengend Snippet: MVD in tumor tissues. (A) The MVD counts for each group were significantly different among the different groups (F=22.660, P<0.001). An obvious increase in MVD was observed in group B (20.970±1.410) compared with that in group A (15.890±1.476, t=7.040, P<0.001), while a significant decrease in MVD was observed in group C (6.366±0.155) compared with that in groups A (t=18.153, P<0.001) and B (t=29.120, P<0.001). (B) Tumor microvessels were identified by cluster of differentiation 31 immunohistochemistry (magnification, ×200). Comparison of the morphology of the tumor vasculature in the three groups revealed that the microvessels in the (a) endostatin-IR-treated group were more regularly distributed and had fewer giant branches than those in the (b) control and (c) IR-treated groups. A, control group; B, IR-treated group; C, endostatin-IR-treated group; IR, ionizing radiation; MVD, microvessel density. ***P<0.001.

Article Snippet: The membranes were blocked with PBS containing 1% Tween 20 at 4°C overnight, washed and incubated overnight at 4°C with anti-HIF-1α (#AH339-2) and anti-endostatin (#BAF1098) primary antibodies (1:1,000 dilution in TBS-T; Bioworld Technology, Inc., St. Louis Park, MN, USA).

Techniques: Immunohistochemistry

Journal: Oncology Letters

Article Title: Efficacy of pEgr-1-endostatin combined with ionizing radiation on hypoxic conditions in nude mice bearing SKOV3 ovarian carcinoma

doi: 10.3892/ol.2017.5559

Figure Lengend Snippet: Expression of HIF-1α in tumor tissues. (A) Agarose gel electrophoresis results of HIF-1α mRNA expression, as evaluated by reverse transcription-polymerase chain reaction in different groups. (B) Relative levels of HIF-1α mRNA in different groups. The difference was statistically significant in the three groups: Groups A and B (t=8.961, P<0.001); groups A and C (t=5.339, P=0.001); groups B and C (t=12.930, P<0.001). (C) The protein level of HIF-1α was analyzed by western blotting. A higher average optical density value for HIF-1α was observed in group B compared with that in groups A and C. The difference was statistically significant (P=0.046). (D) Immunohistochemistry assessment indicated that the expression of HIF-1α was (a) moderate in the control group; (b) strong in the IR-treated group; and (c) weak in the endostatin-IR-treated group (magnification, ×200). A, control group; B, IR-treated group; C, endostatin-IR-treated group; IR, ionizing radiation; M, marker; mRNA, messenger RNA; HIF, hypoxia-inducible factor. ***P<0.001.

Article Snippet: The membranes were blocked with PBS containing 1% Tween 20 at 4°C overnight, washed and incubated overnight at 4°C with anti-HIF-1α (#AH339-2) and anti-endostatin (#BAF1098) primary antibodies (1:1,000 dilution in TBS-T; Bioworld Technology, Inc., St. Louis Park, MN, USA).

Techniques: Expressing, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunohistochemistry, Marker

Journal: Oncology Letters

Article Title: Efficacy of pEgr-1-endostatin combined with ionizing radiation on hypoxic conditions in nude mice bearing SKOV3 ovarian carcinoma

doi: 10.3892/ol.2017.5559

Figure Lengend Snippet: Expression of VEGF in tumor tissues. (A) Agarose gel electrophoresis results of VEGF mRNA expression in tumor tissues, as detected by reverse transcription-polymerase chain reaction. (B) A significant difference was observed in the three groups (F=119.691, P<0.001). Compared with group A (0.530±0.0444), the relative VEGF mRNA expression was increased in group B (0.653±0.0727, P=0.001) but decreased in group C (0.250±0.0359, P<0.001). The expression of VEGF in group C was significantly lower than that in group B (t=14.050, P<0.001). A, control group; B, IR-treated group; C, endostatin-IR-treated group; VEGF, vascular endothelial growth factor; IR, ionizing radiation; mRNA, messenger RNA. **P≥0.001 and <0.05, ***P<0.001.

Article Snippet: The membranes were blocked with PBS containing 1% Tween 20 at 4°C overnight, washed and incubated overnight at 4°C with anti-HIF-1α (#AH339-2) and anti-endostatin (#BAF1098) primary antibodies (1:1,000 dilution in TBS-T; Bioworld Technology, Inc., St. Louis Park, MN, USA).

Techniques: Expressing, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction